Particle Size Analysis Using UV-Vis Spectrophotometer
NanoCuvette™
S
Particle and cell size on your existing UV-Vis, down to 50 nm, with no extra instrument.
The cuvette
Next-gen Scattering Analysis
The NanoCuvette™ S allows for spectrophotometric quantification of particle/cell size and concentration analysis in numerous industries such as biotechnology, pharmaceuticals, life science, foods, including various industrial applications for quality control.
The capacity of NanoCuvette™ S goes far beyond any product currently on the market in terms of achieving easy, fast, and reliable particle/cell size analysis and concentration determination. With the NanoCuvette™ S you have the functionality to determine small particles down to 50 nm, while simultaneously quantify their concentration.
Documentation
Photonic crystal
Expanding Capabilities
The NanoCuvette™ S expands the capabilities of your spectrophotometer and allows you to perform combined angle-resolved light scattering and absorbance at 190–1100 nm. Integrated in the surface is a patented photonic nanocrystal (optical filter), that registers light scattering such that it measures particle sizes near the photonic crystal surface.
Why Upgrade to NanoCuvette™ S
Traditionally, spectrophotometry laboratory work has been limited by lack of robust methods to determine size and concentrations. We upgrade UV-Vis spectrophotometry around the world and across all major instrument brands to create new ways to determine particle size analysis, perform refractive index measurements in a label-free, fast, cheap, and reliable method.
Technology: How We Do It
Absorption versus label-free spectroscopy
With absorption spectroscopy, the Beer–Lambert law relates the attenuation of light to the properties of the material through which the light is traveling:
A = εcl + A0
where A is the absorbance, ε is molar extinction coefficient, c is the concentration, l is the path length and A0 is the background absorbance.
Lambert, J.H. (1760). Photometria sive de mensura et gradibus luminis, colorum et umbrae. Augsburg: Eberhardt Klett. Beer (1852). Bestimmung der Absorption des rothen Lichts in farbigen Flüssigkeiten. Annalen der Physik und Chemie, 162(5), 78–88.
In contrast, when light hits a nanoscale photonic crystal, the resonance wavelength is related to the refractive index or concentration close to the surface:
λ = β(αc + ns) + λ0
where λ is the wavelength, β is a coefficient, α is the specific refractive increment, c is the concentration, ns is the solvent refractive index and λ0 is the reference resonance wavelength, derived from Snell’s law. In this application this is called label-free spectroscopy.
Nano Lett. 2014, 14, 8, 4499–4504. Højlund-Nielsen et al. (2016). Plasmonic Colors: Toward Mass Production of Metasurfaces. Adv. Mater. Technol., 1: 1600054.
Particle Sizing and Concentrations in the Palm of Your Hand.
Key Uses
- Label-free measurements at different volumes (100–200 μL)
- Size analysis of particles from 50 nm to 3000 nm with concentration detection limit of 0.0001% to 0.1%
- Surface refractive index range measurements at 1.0–1.6 nD
- Turbidity and impurity QC measurements
Lab Work Has Never Been Easier
User Centric Experience
With NanoCuvette™ S particles can be quantified in minutes with enhanced sensitivity, reliability, and reproducibility. It measures concentrations and sizes in a standard spectrophotometer without expensive specialized instrument. Together with the unique user-centric online software SpectroWorks™ it automatically analyses the results in minutes.
Furthermore, NanoCuvette™ S has the free “Basic cuvette” spectrum file functionality in SpectroWorks™, which you can use for automatic standard curve processing by using our free report template in our Colab Notebook.
Static Light Scattering and Cloud Computing
Particles can be measured since light scattering depends on angle and wavelength. NanoCuvette™ S utilizes Rayleigh and Mie scattering at any given time to precisely determine the sizes in the dispersion of both large and small particles. SpectroWorks™ uses the scattered light to model the size and concentration within the sample using more than 500 million light simulations.
Superior technology with no Up-front Cost or Training for Your Lab
Key Features
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Supports major brands
Improved and affordable upgrade for your existing UV-Vis spectrophotometer. Also allows basic cuvette measurements.
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Designed for humans
Easy to use with no special training required. Online support included.
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Accurate and instant results
Results are calculated based on spectral analysis. Our engine in SpectroWorks™ is fast and makes it a breeze to extract results.
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Unique traceability
Made in Denmark with unique ID for traceability and access to online calibration data.
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Semi-reusable cuvettes
The NanoCuvettes™ can be re-used up to a certain amount depending on application and maintenance.
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Less sample needed for analysis
Measurements at different volumes between 100–200 μL.
*Purchasing NanoCuvette™ S at a reseller includes lifetime access, data storage and upgrades to SpectroWorks™, free support and individual calibration for best performance. Price per measurement may vary depending on sample and use.
Your Expert in UV-Vis Technology